Viroplasm matrix protein Pns9 from rice gall dwarf virus forms an octameric cylindrical structure

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Viroplasm matrix protein Pns9 from rice gall dwarf virus forms an octameric cylindrical structure.

The non-structural Pns9 protein of rice gall dwarf virus (RGDV) accumulates in viroplasm inclusions, which are structures that appear to play an important role in viral morphogenesis and are commonly found in host cells infected by viruses in the family Reoviridae. Immunofluorescence and immunoelectron microscopy of RGDV-infected vector cells in monolayers, using antibodies against Pns9 of RGDV...

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Hairpin RNA derived from the gene for Pns9, a viroplasm matrix protein of Rice gall dwarf virus, confers strong resistance to virus infection in transgenic rice plants.

The nonstructural Pns9 protein of Rice gall dwarf virus (RGDV) accumulates in viroplasm inclusions, which are structures that appear to play an important role in viral morphogenesis and are commonly found in host cells infected by viruses in the family Reoviridae. An RNA interference construct was designed to target the gene for Pns9 of RGDV, namely Trigger_G9. The resultant transgenic plants a...

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Crystallographic analysis reveals octamerization of viroplasm matrix protein P9-1 of Rice black streaked dwarf virus.

The P9-1 protein of Rice black streaked dwarf virus accumulates in viroplasm inclusions, which are structures that appear to play an important role in viral morphogenesis and are commonly found in viruses in the family Reoviridae. Crystallographic analysis of P9-1 revealed structural features that allow the protein to form dimers via hydrophobic interactions. Each dimer has carboxy-terminal reg...

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Structure of double-shelled rice dwarf virus.

Rice dwarf virus (RDV), a member of the Reoviridae family, is a double-stranded RNA virus. Infection of rice plants with RDV reduces crop production significantly and can pose a major economic threat to Southeast Asia. A 25-A three-dimensional structure of the 700-A-diameter RDV capsid has been determined by 400-kV electron cryomicroscopy and computer reconstruction. The structure revealed two ...

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Rice black streaked dwarf virus P9-1, an a-helical protein, self-interacts and forms viroplasms in vivo

Correspondence Bingsheng Qiu [email protected] Center for Agricultural Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China California Pacific Medical Center Research Institute, San Francisco, CA 94107, USA Bureau of Animal Husbandry and Fishery of Xiangxiang, Xiangxiang 411400, PR China Laboratory of Structural Biology, School of Life Sciences and Bi...

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ژورنال

عنوان ژورنال: Journal of General Virology

سال: 2011

ISSN: 0022-1317,1465-2099

DOI: 10.1099/vir.0.032524-0